Pairs of violet-light-excited fluorochromes for flow cytometric analysis
نویسندگان
چکیده
منابع مشابه
Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode.
BACKGROUND Low power violet laser diodes (VLDs) have been evaluated as potential replacements for water-cooled argon-ion and krypton-ion ultraviolet and violet lasers for DNA content analysis using the Hoechst dyes and 4,6-diamidino-2-phenylindole (Shapiro HMN, Perlmutter NG: Cytometry 44:133-136, 2001). In this study, we used a VLD to excite a variety of violet-excited fluorescent molecules im...
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INTRODUCTION Violet laser diodes have become common and reliable laser sources for benchtop flow cytometers. While these lasers are very useful for a variety of violet and some ultraviolet-excited fluorochromes (e.g., DAPI), they do not efficiently excite most UV-stimulated probes. In this study, the next generation of InGaN near-UV laser diodes (NUVLDs) emitting in the 370-375-nm range have be...
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Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers r...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1998
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19981201)33:4<435::aid-cyto7>3.0.co;2-1